Download "Bioinformatics-Common with Tools in Bioinformatics" Question Paper of B.Sc. (Hons.) Biotechnology for 5th Semester.

B.Sc. (Hons.) Biotechnology

5th Semester 

BIOT-Sem-V-IV-T: Bioinformatics

(Common with Tools in Bioinformatics) 

Time allowed: 3 Hours Max. Marks: 67 

NOTE: Attempt five questions in all, including Question No. 1 which is compulsory, and selecting one question from each Unit.

1. Explain the following briefly: 

a) TREMBL 

b) BLASTN vs BLAST 

c) PSI-BLAST 

d) Log Odd Ratio 

e) Affine Gap Penality 

f) Protein Motifs 

g) Dicotomy trees 

h) Rooted vs Unrooted Trees 

i) mmCIF 

j) Glimmer (10x1-1/2) 

UNIT - I 

II. a) Explain the role of Bioinformatics in the post-genomics era. 

b) Differentiate between 1° and 2° databases? Write a note on GenBank nucleotide sequence database. (6,7) 

III. a) How SCOP and CATH classify the proteins? 

b) Explain how macromolecular structure information is submitted into PDB? (6,7)

UNIT -II 

IV. a) Explain with examples, how Dot Plot can be used to perform sequence alignments. Give merit and demerits of this method.

b) Give various types of basic BLAST. Explain briefly the algorithm of BLAST. (6,7)

V. a) what do you mean by Local Alignment? Explain how Smith Walter-man method. 

b) Explain the Hierarchical method of multiple sequence alignment. (6,7)

UNIT -III

VI. a) Give different tree topologies and terminologies used in molecular phylogeny.

b) Enlist different approaches of phylogenetic tree construction. Explain with example the UPGMA method.  (6,7)

VII a) Write short note on:

i) Maximum Parsimony Method 

ii) GenSan

b) Write a note on gene prediction in the prokaryotic genome. (6,7)

UNIT-IV

VIII. a) Explain the Fold Recognition method of protein tertiary structure prediction. 

b) What are protein 2° structures? Explain Chou Fasman method to predict 2° structures of globular proteins. (6,7) 

IX. a) Explain the concept of Homology Modeling to predict the protein 3D structures. 

b) Give features and applications of Rasmol? (7,6)