Download "Microbial, Physiology Metabolism - 1, Microbiology" Question Paper of B.Sc. (General) for 1st Semester.

B.Sc. (General) First Semester

Microbiology
MIC-102: Microbial, Physiology Metabolism - I 

Time allowed: 3 Hours  Max. Marks: 30

NOTE: Attempt five questions in all, including Question No. I which is compulsory and selecting one question from each Unit.

I. Explain the following:

a) Pychrophiles vs Psychrotrophs 

b) EMB Agar medium 

c) Horizontal gene transfer 

d) Importance of active site of enzymes 

e) Step of conversion of pyruvate to acetyl CoA 

f) Overall reaction of TCA cycle (6x1)

UNIT-I

II. a) Define microbial growth and explain various environmental factors affecting microbial growth.

b) Write down major nutritional types of microorganisms based on energy, electron, and carbon sources.  (2x3)

III. Write note on:

a) Anaerobic growth media 

b) Requirement of N and P in culture media 

c) Importance of MacConky agar medium (3x2)

UNIT –II

IV. Write note on:

a) Hydrolases and Lyases with suitable reactions 

b) Enzyme inhibitors 

c) Effect of substrate concentration on enzyme activity. (3x2)

V. Write note on:

a) Significance of Km value 

b) Covalent modification of enzymes. 

c) Allosteric enzymes(3x2)

UNIT - III

VI. a) Why are fermentation products made during glycolysis? 

b) Flow chart of ED pathway. (2,4)

VII. Write note on:

a) Substrate level phosphorylation 

b) Anaplerotic pathways  (2x3)

UNIT-IV

VIII. Write note on:

a) Role of CAMP in Lac operon 

b) Importance of competence during transformation 

c) Structural genes of tip operon (2x3)

IX. Write note on:- 

a) Interrupted mating experiment

b) Specialized transduction (3,3)