B.Sc. (Hons.) (Bio-Technology) 4th Semester
Animal Biotechnology
Paper: BIOT-Sem-IV
Time Allowed: Three Hours] [Maximum Marks: 67
Note: Attempt five questions in total. Question No. 1 is compulsory.
Attempt one question from each Unit.
1. (a) How gas and nutrient exchange is carried out in organotypic cultures?
(b) Give advantages of embryo transfer.
c) What is the need of cell imaging in the 3D construct of a tissue?
(d) How can animal cells be antigen presenters? What is its use?
e) Give the role of animal virus-based vectors in animal cell transformation.
(f) Which antibiotics can be produced in animal cell lines?
UNIT-I
2. (a) Explain methodology of histotypic cultures and its advantages.
(b) How tissue engineering can be used to revolutionize tissue transplantation?
3. Write notes on:
(i) Structure integrity in organotypic cultures
(ii) Components of tissue engineering and their use.
UNIT-II
4. (a) What is In vitro fertilization? How is IVT carried out in humans?
(b) Which strategies can be used for obtaining vaccines from animal cell cultures?
5. Give different methods for scaling up for anchorage-dependent and anchorage-independent animal cells. Which parameters are to be controlled while scaling up?
UNIT-III
6. (a) How cells containing cloned DNA insert can be selected and screened?
(b) What is transgenic fish? Discuss in brief the procedure of its transgenesis and its applications.
7. Write notes on the following:
(a) Safety regulations in the production of transgenic animals
(b) Methods of transformation of animal cells.
UNIT-IV
8. Give production of interleukins and insulin in animal cell culture.
9. Discuss role of animal biotechnology in the field of medicine with suitable examples.
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