B.Sc. (Hons.) (Biotechnology) 4th Semester
Biophysical and Biochemical Techniques
Paper-BIOT-Sem-IV-II-T
Time Allowed: Three Hours] [Maximum Marks: 67
Note: Attempt five questions in all. Question No. 1 is compulsory. Select one question from each Unit.
(a) What is a Marker Dye in Electrophoresis?
(b) What is the function of Condensor in Microscope?
(C) Define equivalent protons.
(d) What is the principle of Confocal Microscope?
(e) Define swing bucket rotor.
(f) What is a Beta particle?
(g) What is Bragg's Law?
(h) State the principle for GLC.
(i) What is Reverse Phase Chromatography?
(j) Why is denaturing PAGE named so?
UNIT-I
2. (a) Discuss the working of a Fluorimeter.
(b) Discuss the applications of Atomic Absorption Spectroscopy.
3. (a) Discuss the instrumentation for NMR.
(b) Describe the principle and instrumentation for CD Spectroscopy
UNIT-II
4. (a) Discuss the method of specimen preparation for TEM.
(b). Describe the applications of flourescence microscope.
5. (a) Describe the working of bright field microscope.
(b) Describe the technique of differential centrifugation.
UNIT-III
6. (a) Describe the methods and importance of mounting the crystals.
(b) Discuss the role of X-rays in Crystallography.
7. Discuss the different parts of HPLC apparatus. Describe the function of each part as applied in this technique.
UNIT-IV
8. (a) Discuss the properties of different radio-isotopes.
(b) Explain how autoradiography is used for the quantitation of radioactivity.
9. (a) Discuss the principle and applications of GC-MS.
(b) Describe the desorption method of ionisation in MS.
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